Abstract
The draft genome sequence of the thermophilic sulfate-reducing bacterium "Desulfofundulus salinum" strain 435T, isolated from condensate water of the Igrim high-temperature gas field (Western Siberia, Russia), is presented here. The genome is annotated to elucidate the taxonomic position of strain 435T.
Highlights
The draft genome sequence of the thermophilic sulfate-reducing bacterium “Desulfofundulus salinum” strain 435T, isolated from condensate water of the Igrim high-temperature gas field (Western Siberia, Russia), is presented here
Cells were harvested from 2 liters of culture medium by centrifugation after 7 days of incubation, and the cell pellet was resuspended in 400 l of Tris-EDTA (TE) buffer
The dried DNA was dissolved in 50 l of Milli-Q water (MQ)
Summary
The draft genome sequence of the thermophilic sulfate-reducing bacterium “Desulfofundulus salinum” strain 435T, isolated from condensate water of the Igrim high-temperature gas field (Western Siberia, Russia), is presented here. Genomic DNA was extracted using Wilson’s method [11] with minor modifications. Cells were harvested from 2 liters of culture medium by centrifugation after 7 days of incubation, and the cell pellet was resuspended in 400 l of Tris-EDTA (TE) buffer. Thereafter, 25 l of 10% SDS and 20 l of proteinase K solution were added, and the mixture was incubated at 37°C for 60 min.
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