Abstract
Streptococcus suis is primarily a pig pathogen and a zoonotic agent. Recently, the isolation of S. suis strain 10-36905 from a case of meningitis in cattle was reported. The draft genome sequence of this isolate demonstrates its divergent relationship with other S. suis strains.
Highlights
Streptococcus suis is primarily a pig pathogen and a zoonotic agent
A comparison of the genome assembly of 10-36905 to publicly available Streptococcus genomes (n ϭ 622) with Mash (k ϭ 21, s ϭ 1,000,000) (v2.2) [14] distances followed by clustering with the UPGMA within QIIME (v1.9.1) [15] showed that it clustered with S. suis
Proteins common to a set of 73 S. suis and 1 S. parasuis genomes were identified with the LS-BSR tool [16] (v1.0.3) (TBLASTN [17] alignment option), extracted from genome assemblies with TBLASTN, and aligned with MUSCLE (v3.8.31) [18] using the extract_core_genome.py tool within LS-BSR
Summary
Streptococcus suis is primarily a pig pathogen and a zoonotic agent. Recently, the isolation of S. suis strain 10-36905 from a case of meningitis in cattle was reported. Genomic DNA was extracted after culture [8] and sequenced at the University of Wisconsin Biotechnology Center using a MiSeq sequencer and a MiSeq 500-bp (v2) sequencing cartridge, with paired read lengths of 250 bp after library preparation using the TruSeq Nano DNA low-throughput (LT) library prep kit (Illumina). Reads were processed with Skewer (-k, 15; -l, 25) (v0.1.126) [9], and short reads (Ͻ250 nucleotides [nt]) were removed with BBTools (reformat.sh; min length, 250) (v38.61b; https://sourceforge.net/projects/bbmap/).
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