Abstract
ABSTRACTWe report the draft whole-genome sequence of Rothia nasimurium isolated from a porcine tonsil. The genome encodes a nonribosomal peptide synthetase predicted to produce valinomycin, a cyclic dodecadepsipeptide ionophore. Previously, valinomycin was known to be produced only by Streptomyces species and isolates belonging to the Bacillus pumilus group.
Highlights
We report the draft whole-genome sequence of Rothia nasimurium isolated from a porcine tonsil
Genomic DNA was extracted from a culture of Rothia nasimurium isolate that we designated PT-32, grown in brain heart infusion broth (Oxoid, Basingstoke, UK), and sequenced at BaseClear (Leiden, The Netherlands) using an Illumina HiSeq 2500 system on a paired-end library with 125 cycles
Quality assessment was based on Illumina Chastity filtering, removal of reads containing adapters and/or PhiX control signal using a BaseClear in-house filtering protocol, and by using the FASTQC quality control tool version 0.10.0
Summary
We report the draft whole-genome sequence of Rothia nasimurium isolated from a porcine tonsil. Genomic DNA was extracted from a culture of Rothia nasimurium isolate that we designated PT-32, grown in brain heart infusion broth (Oxoid, Basingstoke, UK), and sequenced at BaseClear (Leiden, The Netherlands) using an Illumina HiSeq 2500 system on a paired-end library with 125 cycles. FASTQ sequence files were generated using the Illumina Casava pipeline 1.8.3.
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