Abstract
Sulfate-reducing bacteria are important players in the global sulfur cycle and of considerable commercial interest. The draft genome sequence of a sulfate-reducing bacterium of the family Desulfobacteraceae, assembled from a sulfate-reducing bioreactor metagenome, indicates that heavy-metal– and acid-resistance traits of this organism may be of importance for its application in acid mine drainage mitigation.
Highlights
Sulfate-reducing bacteria (SRB) are widespread and abundant in nature and of considerable commercial importance, ranging from their role in corrosion [1] to their application in sulfate-reducing bioreactors (SRBRs) for treatment of mininginfluenced waters [2]
We present a draft genome of a sulfatereducing bacterium of the family Desulfobacteraceae, which was assembled from a sulfate-reducing bioreactor metagenome
Based on phylogenetic analyses using 16 ribosomal proteins [6], the genome of this organism clustered with Desulfatirhabdium butyrativorans, a butyrate-oxidizing SRB isolated from an anaerobic sludge blanket reactor treating industrial wastewater [7]
Summary
Sulfate-reducing bacteria (SRB) are widespread and abundant in nature and of considerable commercial importance, ranging from their role in corrosion [1] to their application in sulfate-reducing bioreactors (SRBRs) for treatment of mininginfluenced waters [2]. With few exceptions [3, 4], most characterized SRB are not tolerant to acidic conditions typical of acid mine drainage and require circumneutral pH [5] in order to efficiently reduce sulfate and promote metal immobilization in SRBRs. Here, we present a draft genome of a sulfatereducing bacterium of the family Desulfobacteraceae, which was assembled from a sulfate-reducing bioreactor metagenome. The genomic DNA library was prepared using an Illumina TruSEQ DNA library kit and sequenced on an Illumina HiSEQ 2500 paired-end flow cell (2 ϫ 125-bp read length) using V4 Chemistry at the Genomics and Microarray Core, University of Colorado, Denver.
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