Draft genome sequence of a metallo-β-lactamase (blaAIM-1)-producing Klebsiella pneumoniae ST1916 isolated from a patient with chronic diarrhoea.

Abstract

Klebsiella pneumoniae colonisation of the human gastrointestinal tract is a significant risk factor for extraintestinal infections in severely ill patients. Recent reports have indicated the high rate of K. pneumoniae infection resulting from the patient's own gut microbiota. Here we report the draft genome sequence of a multidrug-resistant (MDR) K. pneumoniae strain (SM32) harbouring the blaAIM-1 gene isolated from a patient with chronic diarrhoea in China. Whole genomic DNA was sequenced using an Illumina MiSeq platform. The generated reads were de novo assembled using SOAPdenovo v.2.04. All probable coding sequences were predicted by Glimmer v.3.02 and were annotated using information from GenBank, Pfam, Nr, COG, String, GO and KEGG. Resistance-related genes were also further identified. The draft genome of K. pneumoniae SM32, belonging to sequence type (ST) 1916, was assembled into 165 contigs comprising 5238542bp. A total of 5013 protein-coding sequences and several genes associated with resistance to β-lactams, tetracycline, aminoglycosides, fluoroquinolones and trimethoprim/sulfamethoxazole were preliminarily identified. MDR K. pneumoniae colonising the human gastrointestinal tract provides a potential reservoir for extraintestinal infections. The genome sequence of K. pneumoniae SM32 will be helpful to reveal the key role of mobile genetic elements in the adaptive translocation and spread of antimicrobial resistance.