Abstract
Here, we report the genome sequence of Tsukamurella tyrosinosolvens strain PS2, which was isolated from hydrocarbon sludge of an organic synthesis factory. This strain was able to utilize a wide range of n-alkanes, from C16 to C35, as sole carbon sources. Knowledge of the genome will provide insights into long-chain n-alkane biodegradation mechanisms.
Highlights
We report the genome sequence of Tsukamurella tyrosinosolvens strain PS2, which was isolated from hydrocarbon sludge of an organic synthesis factory
Genomic DNA was extracted from pure culture grown on LB medium according to a miniprep protocol, with detergent lyses and phenol-chloroform purification performed as described by Wilson [11]
The quantity of extracted DNA was measured with a Qubit 2.0 fluorometer (Invitrogen)
Summary
We report the genome sequence of Tsukamurella tyrosinosolvens strain PS2, which was isolated from hydrocarbon sludge of an organic synthesis factory. Genomic DNA was extracted from pure culture grown on LB medium according to a miniprep protocol, with detergent lyses and phenol-chloroform purification performed as described by Wilson [11]. After fragmentation of genomic DNA by a Covaris ultrasonication system, the DNA library was constructed with a NEBNext Ultra library preparation kit, according to the manufacturer’s instructions. The quality of the resulting library was estimated using an Agilent 2100 Bioanalyzer.
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