Draft Genome Sequence of a Dictyoglomus sp. from an Enrichment Culture of a New Zealand Geothermal Spring

Anna-Louise Reysenbach,John F Kelley,John A Donaho,Emily St John,Matthew B Stott,Mircea Podar,Christina Turner

doi:10.1128/genomea.00150-18

Abstract

ABSTRACTA draft genome of a novel Dictyoglomus sp., NZ13-RE01, was obtained from a New Zealand hot spring enrichment culture. The 1,927,012-bp genome is similar in both size and G+C content to other Dictyoglomus spp. Like its relatives, Dictyoglomus sp. NZ13-RE01 encodes many genes involved in complex carbohydrate metabolism.

Highlights

A draft genome of a novel Dictyoglomus sp., NZ13-RE01, was obtained from a New Zealand hot spring enrichment culture
We obtained a genome of a novel Dictyoglomus sp., NZ13-RE01, from a New Zealand hot spring enrichment culture (Hell’s Gate, Tikitere, New Zealand, 38°03=47ЉS, 176°21=39ЉE, pH 6.0, 74°C)
Open reading frames were annotated using the Rapid Annotations using Subsystems Technology (RAST) server [22,23,24], the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) [25], the Clusters of Orthologous Groups of proteins (COG) database [26], and the dbCAN database [27]. tRNAs were predicted with tRNAscan-SE version 2.0 [28]

Summary

Introduction

A draft genome of a novel Dictyoglomus sp., NZ13-RE01, was obtained from a New Zealand hot spring enrichment culture. We obtained a genome of a novel Dictyoglomus sp., NZ13-RE01, from a New Zealand hot spring enrichment culture (Hell’s Gate, Tikitere, New Zealand, 38°03=47ЉS, 176°21=39ЉE, pH 6.0, 74°C). Spherical bodies were apparent in the enrichment cultures by phasecontrast microscopy. DNA was extracted using the Qiagen DNeasy blood and tissue kit. Metagenome Nextera DNA libraries were sequenced on the Illumina MiSeq platform.

Results

Conclusion