Draft Genome Sequence of a Bacillus Bacterium from the Atacama Desert Wetlands Metagenome.

Claudia Vilo,Benito Gómez-Silva,Alexandra Galetovic,Jorge E Araya,Qunfeng Dong

doi:10.1128/genomea.00955-15

Claudia Vilo, Benito Gómez-Silva + Show 3 more

Open Access

https://doi.org/10.1128/genomea.00955-15

Copy DOI

Abstract

We report here the draft genome sequence of a Bacillus bacterium isolated from the microflora of Nostoc colonies grown at the Andean wetlands in northern Chile. We consider this genome sequence to be a molecular tool for exploring microbial relationships and adaptation strategies to the prevailing extreme conditions at the Atacama Desert.

Highlights

Colonies of a Nostoc cyanobacterium grow naturally at wetlands Ͼ3,000 m of altitude at the Andes Mountains range in South America
We isolated a Bacillus bacterium from the complex microbiota associated with Llayta colonies and decided to sequence its genome in order to have a molecular tool to address physiological relationships between the dominant Llayta cyanobacterium and its associated microbiome. It will allow the exploration of microbial survival strategies to extreme dryness, arsenic, heavy metals, UV radiation, and other prevailing extreme environmental conditions in the Atacama Desert [3]
We sequenced the Bacillus species genome via MiSeq sequencing technology using paired-end libraries, with an average insert size of 250 bp

Summary

Introduction

Colonies of a Nostoc cyanobacterium grow naturally at wetlands Ͼ3,000 m of altitude at the Andes Mountains range in South America. We isolated a Bacillus bacterium from the complex microbiota associated with Llayta colonies and decided to sequence its genome in order to have a molecular tool to address physiological relationships between the dominant Llayta cyanobacterium and its associated microbiome. It will allow the exploration of microbial survival strategies to extreme dryness, arsenic, heavy metals, UV radiation, and other prevailing extreme environmental conditions in the Atacama Desert [3]. We sequenced the Bacillus species genome via MiSeq sequencing technology using paired-end libraries, with an average insert size of 250 bp.

Results

Conclusion