Draft Genome Sequence for the Type Strain Vulcanibacillus modesticaldus BR, a Strictly Anaerobic, Moderately Thermophilic, and Nitrate-Reducing Bacterium Isolated from Deep-Sea Hydrothermal Vents of the Mid-Atlantic Ridge.

Christopher A Abin,James T Hollibaugh

doi:10.1128/genomea.01246-16

Abstract

Vulcanibacillus modesticaldus BRT was isolated from calcite-rich, metalliferous core samples collected at the Rainbow deep-sea hydrothermal vent field on the Mid-Atlantic Ridge. Here, we report the 2.2-Mb draft genome sequence for this strain, consisting of 100 contigs with a G+C content of 33.6% and 2,227 protein-coding sequences.

Highlights

Vulcanibacillus modesticaldus BRT was isolated from core samples collected at the Rainbow hydrothermal vent field on the Mid-Atlantic Ridge (MAR) (36°14= N, 33°54= W) [1]
The Rainbow vent field is located at a depth of ~2,300 m on the western flank of a nonvolcanic ridge of the MAR, southwest of the Azores Archipelago [2]
Nitrite produced from dissimilatory nitrate reduction is not reduced further to ammonium or to N2, indicating that the strain is unable to perform dissimilatory nitrate reduction to ammonium (DNRA) or denitrification [1]

Summary

Introduction

Vulcanibacillus modesticaldus BRT was isolated from core samples collected at the Rainbow hydrothermal vent field on the Mid-Atlantic Ridge (MAR) (36°14= N, 33°54= W) [1]. V. modesticaldus BRT was able to grow at temperatures from 37 to 60°C, pH values of 6.0 to 8.5, and salinities of 1 to 4%. The strain grew chemoheterotrophically with organic acids, carbohydrates, and complex proteinaceous substrates as electron donors with nitrate as the sole electron acceptor. Nitrite produced from dissimilatory nitrate reduction is not reduced further to ammonium or to N2, indicating that the strain is unable to perform dissimilatory nitrate reduction to ammonium (DNRA) or denitrification [1].

Results

Conclusion