Abstract

Clostridium thermocellum is a potent cellulolytic bacterium. C. thermocellum strain PAL5, was derived from strain S14 that was isolated from bagasse paper sludge, possesses higher cellulose-degradation ability than representative strains ATCC27405 and DSM1313. In this work, we determined the draft genome sequence of C. thermocellum PAL5. Genomic DNA was used for whole-genome sequencing using the Illumina HiSeq 2500. We obtained 215 contigs of >200 bp (N50, 78,366 bp; mean length, 17,378 bp). The assembled data were subjected to the National Center for Biotechnology Information (NCBI) Prokaryotic Genome Annotation Pipeline, and 3198 protein-coding sequences, 53 tRNA genes, and 4 rRNA genes were identified. The data are accessible at NCBI (the accession number SBHL00000000). Our data resource will facilitate further studies of efficient cellulose-degradation using C. thermocellum.

Highlights

  • Eiko Nakazono-Nagaoka a, Takashi Fujikawa b, Ayumi Shikata a, Chakrit Tachaapaikoon c, Rattiya Waeonukul c, Patthra Pason c, Khanok Ratanakhanokchai d, Akihiko Kosugi a, *

  • C. thermocellum strain PAL5, was derived from strain S14 that was isolated from bagasse paper sludge, possesses higher cellulosedegradation ability than representative strains ATCC27405 and DSM1313

  • The assembled data were subjected to the National Center for Biotechnology Information (NCBI) Prokaryotic Genome Annotation Pipeline, and 3198 protein-coding sequences, 53 tRNA genes, and 4 rRNA genes were identified

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Summary

Introduction

Eiko Nakazono-Nagaoka a, Takashi Fujikawa b, Ayumi Shikata a, Chakrit Tachaapaikoon c, Rattiya Waeonukul c, Patthra Pason c, Khanok Ratanakhanokchai d, Akihiko Kosugi a, *. Draft genome sequence data of Clostridium thermocellum PAL5 possessing high cellulosedegradation ability We determined the draft genome sequence of C. thermocellum PAL5.

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