Abstract
Cercospora kikuchii (Tak. Matsumoto & Tomoy.) M.W. Gardner 1927 is an ascomycete fungal pathogen that causes Cercospora leaf blight and purple seed stain on soybean. Here, we report the first draft genome sequence and assembly of this pathogen. The C. kikuchii strain ARG_18_001 was isolated from soybean purple seed collected from San Pedro, Buenos Aires, Argentina, during the 2018 harvest. The genome was sequenced using a 2 × 150 bp paired-end method by Illumina NovaSeq 6000. The C. kikuchii protein-coding genes were predicted using FunGAP (Fungal Genome Annotation Pipeline). The draft genome assembly was 33.1 Mb in size with a GC-content of 53%. The gene prediction resulted in 14,856 gene models/14,721 protein coding genes. Genomic data of C. kikuchii presented here will be a useful resource for future studies of this pathosystem. The data can be accessed at GenBank under the accession number VTAY00000000 https://www.ncbi.nlm.nih.gov/nuccore/VTAY00000000.
Highlights
The isolation technique is described in [4]. This strain was deposited in the fungal culture collection of the Department of Plant Pathology, School of Agriculture, University of Buenos Aires (FAUBA, Argentina)
The repetitive regions, including tandem repeats and transposable elements, were detected using the repeat identification tool RepeatMasker [21]
Summary
Cercospora kikuchii strain ARG_18_001 was isolated from a single conidium from soybean seeds of variety DM62R63 sampled that exhibited symptoms of purple seed stain during the 2018 harvest in San Pedro, Buenos Aires, Argentina. The isolation technique is described in [4]. This strain was deposited in the fungal culture collection of the Department of Plant Pathology, School of Agriculture, University of Buenos Aires (FAUBA, Argentina). The DNA extraction was carried out at the Institute of Microbiology and Agricultural Zoology (IMYZA -INTA) using a modified. Cetyltrimethylammonium bromide (CTAB) extraction protocol developed by [5]. Paired-end whole-genome shotgun libraries were constructed using the TruSeq Nano DNA (insert size 350 bp) library preparation kit following Illumina (San Diego, CA) protocols. Sequencing was performed using a NovaSeq 6000 sequencing system (Illumina) and yielded 65,202,278 reads
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