Abstract
Mosses comprise one of three lineages forming a sister group to extant vascular plants. Having emerged from an early split in the diversification of embryophytes, mosses may offer complementary insights into the evolution of traits following the transition to, and colonization of, land. Here, we report the draft nuclear genome of Fontinalis antipyretica (Fontinalaceae, Hypnales), a charismatic aquatic moss that is widespread in temperate regions of the Northern Hemisphere. We sequenced and de novo-assembled its genome using the 10X Genomics method. The genome comprises 385.2Mbp, with a scaffold N50 of 45.8Kbp. The assembly captured 87.2% of the 430 genes in the BUSCO Viridiplantae odb10 dataset. The newly generated F. antipyretica genome is the third moss genome, and the second seedless aquatic plant genome, to be sequenced and assembled to date.
Highlights
Mosses comprise one of three lineages forming a sister group to extant vascular plants
The early diversification of land plants is marked by various morphological innovations, such as branching of the sporophyte or stomata [6], as well as metabolic innovations – notably biopolymers, essential materials for cuticle composition [7], which enable plants to adapt to a water-deficient, UV-exposed living environment
Sequencing the genome of F. antipyretica should provide the first opportunity for a comparative genomic study in this lineage, which may have diversified after the rise of the angiosperms
Summary
Mosses comprise one of three lineages forming a sister group to extant vascular plants. A protocol collection including methods for BGISEQ-500 and 10X Genomics library construction and sequencing is available via protocols.io (Figure 2). The remaining reads were subsequently de novo-assembled using 10X Genomics Supernova v2.1.1 (RRID:SCR_016756) with default parameters [11].
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