Draft genome of Streptomyces sp. strain 130 and functional analysis of extracellular enzyme producing genes.

Abstract

Streptomyces sp. strain 130 possesses multiple uncharacterized extracellular enzyme producing genes. Enzymes from these genes may fulfil the intense demand of stable and effective extracellular enzymes in various industries. Taxonomy of Streptomyces sp. strain 130 was validated by FAME analysis. Strain 130 was screened for the presence of chitinase producing genes of family 18 and 19 using SC1F/SC2R and F19F2/F19R primer sets respectively. Whole genome sequencing was done using Illumina Next Seq500 system. In the analysis of draft genome of Streptomyces sp. strain 130, the genome size was found to be 7.1Mb. Blastn and NCBI-conserved domain search tool were used to find similarity percentage with genes in existing database and enzyme family respectively. Ten chitinase, six xylanase and one cellulase producing genes were present in draft genome. Among the ten chitinase producing genes, two were belonging to GH19 family and other eight to GH18 family chitinase. Six out of ten chitinase producing genes were uncharacterized and one belonged to family GH18_PF-ChiA (PF-ChiA is a chitinase found in the hyperthermophilic archaea, prokaryotes). In case of xylanase, four out of six (GH9, 43, 10 and 11 enzyme family) were not showing nucleotide based similarity with any characterized gene. The study of reported genome sequence will help us to identify gene sequence of characterized and uncharacterized extracellular enzyme producing genes. Cloning of each gene and enzyme activity assay of their products will reveal the activity and stability at different variables; and resulting products may have huge applications at industrial scale.