Draft genome of Gemmata massiliana sp. nov, a water-borne Planctomycetes species exhibiting two variants.

Rita Aghnatios,Catherine Robert,Marc Garibal,Caroline Cayrou,Said Azza,Didier Raoult,Michel Drancourt

doi:10.1186/s40793-015-0103-0

Abstract

Gemmata massiliana is a new Planctomycetes bacterium isolated from a hospital water network in France, using a new culture medium. It is an aerobic microorganism with optimal growth at pH 8, at 30 °C and salinity ≤ 1.25 % NaCl. G. massiliana is resistant to β-lactam antibiotics, due to lack of peptidoglycan in its cell wall.G. massiliana shares a 97 % 16S rRNA gene sequence similarity with the nearest species, Gemmata obscuriglobus; and 99 % similarity with unnamed soil isolates. Its 9,249,437-bp genome consists in one chromosome and no detectable plasmid and has a 64.07 % G + C content, 32.94 % of genes encoding for hypothetical proteins. The genome contains an incomplete 19.6-kb phage sequence, 26 CRISPRs, 3 CAS and 15 clusters of secondary metabolites. G. massiliana genome increases knowledge of a poorly known world of bacteria.Electronic supplementary materialThe online version of this article (doi:10.1186/s40793-015-0103-0) contains supplementary material, which is available to authorized users.

Highlights

Gemmata obscuriglobus, the sole cultured representative of the genus Planctomycetes Gemmata, was first isolated in a freshwater dam in Queensland, Australia [1]
Antibiotics were added to the culture medium for a selective isolation of Planctomycetes which are broadly resistant to antibiotics [21]
Genome project history The organism was selected for sequencing on the basis of its phylogenetic position and 16S rRNA gene similarity to G. obscuriglobus, the sole named species in this genus, and its isolation was done in the context of a study on the detection of Planctomycetes bacteria in the hospital water network

Summary

Introduction

The sole cultured representative of the genus Planctomycetes Gemmata, was first isolated in a freshwater dam in Queensland, Australia [1]. Genome project history The organism was selected for sequencing on the basis of its phylogenetic position and 16S rRNA gene similarity to G. obscuriglobus, the sole named species in this genus, and its isolation was done in the context of a study on the detection of Planctomycetes bacteria in the hospital water network. G. massiliana had a slightly larger genome than G. obscuriglobus (9.249 Mb vs 9.16 Mb), a lower G + C content (64 % vs 67.2 %), it codes for a higher number of genes (8,065 vs 7,645), had six cell-shape and division proteins in common with G. obscuriglobus and the other Planctomycetes previously studied [30] and four detected for the first time in this phylum. Showed a different antibiotic resistance profile [21]

Conclusions

Findings

1.24 Intracellular trafficking and secretion