DPPH Free-radical Scavenging and Cytotoxic Activities of Leeamacrophylla

A B M Faroque,Dilruba Akter,Firoj Ahmed,Md Abdul Muhit,Sheikh Zahir Raihan

doi:10.3329/bmrcb.v44i2.38690

A B M Faroque, Dilruba Akter + Show 3 more

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https://doi.org/10.3329/bmrcb.v44i2.38690

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Abstract

Structural damage inflicted to a wide variety of bioorganic compounds (DNA, protein, carbohydrates, and lipid) may occur as a consequence of oxidative damage which is naturally attributed to our daily lifestyle. A number of mechanisms are exploited to neutralize the reactive oxygen species. Medicinal plant can play a pivotal role of detoxifying the generated ROS. Leeamacrophylla (Leeaceae), a wild medicinal plant, has been taken into consideration of having antioxidant and cytotoxic properties. In this study, in vitro DPPH free radical scavenging activity, total phenolic content and total flavonoid content of methanol extract from the leaves of L. macrophylla were determined with using UV spectrophotometric method. Antioxidant activity of the crude extract was expressed as percentage of DPPH free radical inhibition and IC50 value (μg/ml). The IC50 value of L. macrophylla was found to be 152.40μg/ml. Compared to standard ascorbic acid (4.36 μg/ml), L. macrophylla leaf extract has moderate antioxidant property. The total phenolic content was 65.75 mg/g of dry weight of extract, expressed as gallic acid equivalents. The total flavonoid concentration was 59.8 mg/g, expressed as quercetin equivalents. In the brine shrimp lethality test, LC50value of crude methanolic extract obtained from the best-fit line slope was 3.06μg/ml which was significant comparing with control (vincristine sulphate). Above results suggested that the L. macrophylla can be regarded as a promising candidate for natural plant sources of antioxidants with high value.

Highlights

Oxidative stress is closely related with the pathogenesis of life-style diseases such as atherosclerosis, hypertension, diabetes mellitus, ischemic diseases, and malignancies.[1]
The way of determination of the total flavonoid content was done on the basis of absorbance value of crude methanolic extract reacting with aluminum chloride
The total flavonoid content of the crude methanolic extract, compared with calibration curve (R2=0.993), was 59.8±1.45 quercetin equivalents/g. Phenolic compounds such as tannins, catechins and flavonoids have a number of hydroxyl groups which have redox properties, which allows them to act as antioxidants

Summary

Introduction

Oxidative stress is closely related with the pathogenesis of life-style diseases such as atherosclerosis, hypertension, diabetes mellitus, ischemic diseases, and malignancies.[1]. The roots of the plant have been used traditionally in fracture and healing cut injury and to allay pain. It has astringent, styptic and antiseptic activity.[7] Potential anti-inflammatory effect of L. macrophylla leaves has been reported in experimental rats.[8] Previously, researchers have reported antinociceptive and antioxidant activities of different extracts of roots of L. macrophyllain swiss albino.[9,10] So far, there are no scientific reports about antioxidant and cytotoxicity potentials of the leaves of L. macrophylla in the country

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