Doxycycline-Loaded Nitric Oxide-Releasing Nanomatrix Gel in Replanted Rat Molar on Pulp Regeneration.

Kwan-Hee Yun,Ok-Hyung Nam,Sung-Chul Choi,Koeun Lee,Hyo-Seol Lee,Kyounga Cheon,Mi-Ja Ko,Yong-Kown Chae

doi:10.3390/app11136041

Abstract

The aim of the present study was to evaluate the effect of doxycycline-loaded NO-releasing nanomatrix gel on pulp regeneration in replantation of avulsed rat teeth. A total of 28 maxillary first molars extracted from rats were replanted. The rats were divided into two groups based on the use of root surface treatment: doxycycline-loaded NO-releasing nanomatrix group and no treatment. Eight weeks after replantation, the rats were sacrificed, and the teeth were evaluated using histomorphometric analysis. On histomorphometric analysis, the NO-releasing nanomatrix group demonstrated a significantly lower grade of pulp inflammation (1.00 ± 1.11, mean ± standard deviation) compared to the no treatment group (2.21 ± 1.25, p = 0.014). NO-releasing nanomatrix group showed a significantly higher grade of pulp regeneration (2.57 ± 0.85, p = 0.012) and significantly lower grade of pulp inflammation (1.00 ± 0.68, p = 0.025) compared to the no treatment group. In conclusion, NO-releasing nanomatrix gel improved pulp regeneration of replanted teeth, though the sample size of this study was rather small. Within the limits of this study, NO-releasing nanomatrix gel can provide more favorable pulpal regeneration despite replantation.

Highlights

Published: 29 June 2021Tooth avulsion is a complete displacement of a tooth from its alveolar socket due to a traumatic dental injury (TDI) [1]
Nitric oxide (NO)-releasing nanomatrix gel was evaluated for pulp regeneration in replantation of was significantly increased compared to Group II
Because the purpose of this study was to evaluate regenerative potenwas observed in Group I of replantation after 60 min storage

Summary

Introduction

Published: 29 June 2021Tooth avulsion is a complete displacement of a tooth from its alveolar socket due to a traumatic dental injury (TDI) [1]. With tooth avulsion and replantation procedures, the damaged root surface structure and periodontium undergo inflammatory and infectious stages, often leading to a failure in functional recovery [2,3]. It is recommended that the avulsed tooth should be replanted as soon as possible after the trauma and stabilized to allow healing [2,4]. For the replantation of an avulsed tooth, treatment goals are to promote healing without inflammation or infection and to promote the regeneration of the tooth’s apex structure. Topical application of doxycycline to the root surface prior to replantation has been suggested and utilized to control microorganisms and minimize inflammatory responses [5,8]. Application of doxycycline prior to replantation has been reported to induce pulp regeneration, but complete regeneration of pulp tissue by doxycycline has not been achieved yet [5,6,9,10]

Objectives

Methods

Results

Conclusion