Doxycycline Increases Absence-like Epileptic Seizures in WAG/Rij Rats

Abstract

Event Abstract Back to Event Doxycycline Increases Absence-like Epileptic Seizures in WAG/Rij Rats Z. Kovács1*, A. K. Kékesi2, G. Juhász2 and A. Czurkó3 1 University of West Hungary, Department of Zoology, Hungary 2 Eötvös Loránd University, Hungary 3 University of Szeged, Institute of Medical Chemistry, Hungary Epileptic seizures might activate matrix metalloproteinases (MMPs) in the brain and increased MMP-9 activity have been found in a genetic model of generalized absence epilepsy (Wistar Albino Glaxo Rijswijk/WAG/Rij rats). In this study we investigated the effect of subantimicrobial doses of MMP inhibitor doxycycline on spike-wave-discharges (SWDs) in WAG/Rij rats. A single dose of 20 mg/kg doxycycline were injected intraperitoneally (i.p.) into WAG/Rij rats and number and duration of SWDs were measured for 4 hours. We found that 20 mg/kg doxycycline significantly increased the incidence and duration of SWDs compared to the preceding three-day average control level during the whole four-hour recording period. Doxycycline at similar dosage inhibits also inflammation, e.g. systemic injection of 10 mg/kg of doxycycline blocks the increase of pro-inflammatory cytokines. While the role of inflammation in absence epilepsy is not elucidated yet, we also investigated the effect of 10 mg/kg doxycycline on the number of SWDs. We found that 10 mg/kg doxycycline also increased the number of SWD but only between 30-90 min after i.p. injection. Our results indicate that while in many epilepsy models the overexpression of MMP-9 could deteriorate the behavioral seizures and its consequences, in case of absence seizures MMP-9 may exert beneficial effects. Keywords: disorders, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Disorders Citation: Kovács Z, Kékesi AK, Juhász G and Czurkó A (2011). Doxycycline Increases Absence-like Epileptic Seizures in WAG/Rij Rats. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00163 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. Z. Kovács, University of West Hungary, Department of Zoology, Szombathely, Hungary, kovacs@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Z. Kovács A. K Kékesi G. Juhász A. Czurkó Google Z. Kovács A. K Kékesi G. Juhász A. Czurkó Google Scholar Z. Kovács A. K Kékesi G. Juhász A. Czurkó PubMed Z. Kovács A. K Kékesi G. Juhász A. Czurkó Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.