Abstract
Recent evidence implicates a particular role for the serotonergic system in regulation of adult neurogenesis in a mouse animal model of hippocampus. Serotonin (5-HT) serves as a protector of adult neural stem cell (ANSC) growth. Lack of 5-HT caused by tryptophan-hydroxylase (TPH) depletion leads to a decrease in stem cell proliferation. Both isoforms of TPH (TPH1 and TPH2) are present in undifferentiated ANSC. So far, no reliable TPH1 and TPH2 knockout animal models are available; i.e. TPH2 KO mice are infertile. Therefore, we sought to investigate the influence of different TPH isoforms in-vitro by a selective gene-silencing approach. In addition to the lack of a suitable animal model, we sought to employ the novel technique of RNA interference in order to study TPH 1 and 2 isoforms on the particularly interesting level of adult neurogenesis.
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