Abstract
The Escherichia coli guaB promoter (PguaB) regulates transcription of two genes, guaB and guaA, that are required for the synthesis of guanosine 5′-monophosphate (GMP), a precursor for the synthesis of guanine nucleoside triphosphates. Transcription from PguaB increases as a function of increasing cellular growth rate, and this is referred to as growth rate-dependent control (GRDC). Here we investigated the role of the factor for inversion stimulation (FIS) in the regulation of this promoter. The results showed that there are three binding sites for FIS centred near positions −11, +8 and +29 relative to the guaB transcription start site. Binding of FIS to these sites results in repression of PguaB in vitro but not in vivo. Deletion of the fis gene results in increased PguaB activity in vivo, but GRDC of PguaB is maintained.
Highlights
The Escherichia coli guaB promoter (PguaB) regulates transcription of two genes, guaB and guaA, which together constitute the guaBA operon
PguaB is regulated by the cAMP receptor protein (CRP) and a putative CRP binding site is centred near position 2117.5 relative to the guaB transcription start site (Hutchings & Drabble, 2000)
Category 1 sites contain bases that match the consensus at 4/5 critical positions, including the outer bases that are most strongly conserved among FIS sites and which are presumed to be bound by the D helices of FIS (Shultzaberger et al, 2007)
Summary
The Escherichia coli guaB promoter (PguaB) regulates transcription of two genes, guaB and guaA, which together constitute the guaBA operon. It has been shown that the rate of transcription from PguaB per unit cell mass increases as a function of increasing cellular growth rate (Davies & Drabble, 1996; Husnain & Thomas, 2008). This phenomenon is commonly referred to as growth ratedependent control (GRDC) (Gourse et al, 1996; Dennis et al, 2004). GRDC of PguaB requires the UP element and sequences located upstream of the UP element (Husnain & Thomas, 2008)
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