Abstract
The objective of this study was to observe the infection of human cytomegalovirus (HCMV) to human umbilical vein endothelial cells, and its effect on the expression of single-stranded DNA-binding protein (SSBP1) and on lipid metabolism in endothelial cells. We screened the differential expression of mRNAs after HCMV infection by suppression subtractive hybridization and the expression levels of SSBP1 mRNA and protein after HCMV infection by real-time PCR and western blot. After verification of successful infection by indirect immunofluorescent staining and RT-PCR, we found a differential expression of lipid metabolism-related genes including LDLR, SCARB, CETP, HMGCR, ApoB and LPL induced by HCMV infection. The expression levels of SSBP1 mRNA and protein after HCMV infection were significantly down-regulated. Furthermore, we found that upregulation of SSBP1 inhibited the expression of atherosclerosis-associated LDLR, SCARB, HMGCR, CETP as well as the accumulation of lipids in the cells. The results showed that the inhibition of SSBP1 by HCMV infection promotes lipid accumulation in the cells.
Highlights
Most of the viral infections humans encounter during their life are effectively cleared by the immune system
Infection of human umbilical vein endothelial cells (HUVECs) by human cytomegalovirus (HCMV) The results showed the swelling of some individual cells after 24 h of infection
These results showed that HCMV successfully infected HUVECs
Summary
Most of the viral infections humans encounter during their life are effectively cleared by the immune system. Examples of such viruses are influenza and respiratory syncytial virus. Like human cytomegalovirus (HCMV) and Epstein Barr virus, and other viruses like HIV persist after the primary infection, hiding or constantly escaping from the immune system. It is a DNA virus that belongs to the herpes virus family type 5. It can infect any population, with 80% of infection rate before the age of 3, up to 100% of infection rate in adulthood. HCMV is thought to be closely related to lipid metabolism disorders and plays an important role in the pathogenic mechanism of various diseases, especially atherosclerosis (AS) [1]
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