Abstract
BackgroundWe found that selenium-binding protein 1 (SBP1) was progressively decreased in the human bronchial epithelial carcinogenic processes. Knockdown of SBP1 in immortalized human bronchial epithelial cell line 16HBE cells significantly increased the efficiency of B[a]P-induced cell transformation. However, the relationship between SBP1 expression and clinicopathological factors of patients has not been defined completely. The specific role of SBP1 in prognosis of lung squamous cell carcinoma (LSCC) is still unknown.MethodsTissue samples from 82 patients treated by pulmonary lobectomy for LSCC were used. Immunohistochemistry and western blotting were used to detect the expressions of SBP1 protein. The relationships between the expression level of SBP1 and the clinicopathological features of patients were analyzed. Cox proportional hazard regression analysis and Kaplan–Meier method were used to perform survival analysis.ResultsExpressions of SBP1 proteins were significantly lower in LSCC tissues than that in the corresponding normal bronchial epithelium (NBE) tissues (P = 0.000). In LSCC, The expression levels of SBP1 had not correlated with patients’ age, gender, smoking state, primary tumor stages (T), TNM clinical stages, and distant metastasis (M) (P > 0.05). However, downregulation of SBP1 was significantly associated with higher lymph node metastasis and lower overall survival rate (P < 0.05). Cox regression analysis indicated low expressions of SBP1 can be an independent prognostic factor for poor overall survival in LSCC patients (P = 0.002).ConclusionsDownregulation of SBP1 may play a key role in the tumorigenic process of LSCC. SBP1 may be a novel potential prognostic factor of LSCC.
Highlights
We found that selenium-binding protein 1 (SBP1) was progressively decreased in the human bronchial epithelial carcinogenic processes
Immunohistochemical analysis demonstrated that SBP1 protein expression decreased significantly in lung squamous cell carcinoma (LSCC) compared to its abundance in the corresponding normal bronchial epithelium (NBE)
The expressional levels of SBP1 protein were further verified by western blotting analysis, which were performed with 16 pairs of microdissected fresh LSCC and matched NBE tissues
Summary
We found that selenium-binding protein 1 (SBP1) was progressively decreased in the human bronchial epithelial carcinogenic processes. The specific role of SBP1 in prognosis of lung squamous cell carcinoma (LSCC) is still unknown. SCC is still the most common histologic type of primary lung cancers in developing countries, its ratio has decreased while that of adenocarcinoma increased over the years [6]. The expressions of SBP1 were reported to decrease markedly in numerous tumor types compared with their corresponding normal tissues. We had found that SBP1 was progressively decreased in the human bronchial epithelial carcinogenic processes and SBP1 expression could distinguish normal bronchial epithelium (NBE) from preneoplastic lesions and invasive LSCC. In this study, we investigated the expression of SBP1 in LSCC and corresponding NBE tissues by immunohistochemistry and western blotting, evaluated the relationship of SBP1 expression and clincopathological factors, and further determined its prognostic significance via analyzing the correlation of SBP1 expression with survival
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