Abstract
Aim:To determine the expression patterns of the RBBP6 spliced variants during arsenic trioxide-mediated cell cycle arrest and curcumin-induced apoptosis in MCF-7 cells.Materials & methods:As2O3 and curcumin were used to study cytotoxicity, cell cycle arrest, apoptosis and the expression of RBBP6 variants. The MUSE Cell Analyser was used to analyze cell cycle arrest, apoptosis and multicaspase activity while apoptosis was further confirmed using microscopy. Semi-quantitative RT-PCR was employed to quantitate the expression of the RBBP6 variants.Results:This study showed that the MCF-7 cells expressed RBBP6 variant 1 but lacked both variant 2 and variant 3. Both As2O3 and curcumin significantly downregulated RBBP6 variant 1 (p < 0.001).Conclusion:RBBP6 variants are promising therapeutic targets.
Highlights
RBBP6 variant 3 is against the carcinogenesis process and it is downregulated in MCF-7 cells
Arsenic trioxide upregulated the expression of Bax protein in MCF-7 breast cancer cells
Our study suggests that there are RBBP6 variants that are procarcinogenic and there are those that are anticarcinogenic
Summary
To determine the expression patterns of the RBBP6 spliced variants during arsenic trioxide-mediated cell cycle arrest and curcumin-induced apoptosis in MCF-7 cells
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