Downregulation of miR-503 Promotes ESCC Cell Proliferation, Migration, and Invasion by Targeting Cyclin D1

Lanfang Jiang,Zitong Zhao,Leilei Zheng,Liyan Xue,Qimin Zhan,Yongmei Song

doi:10.1016/j.gpb.2017.04.003

Abstract

Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive cancers in China, but the underlying molecular mechanism of ESCC is still unclear. Involvement of microRNAs has been demonstrated in cancer initiation and progression. Despite the reported function of miR-503 in several human cancers, its detailed anti-oncogenic role and clinical significance in ESCC remain undefined. In this study, we examined miR-503 expression by qPCR and found the downregulation of miR-503 expression in ESCC tissue relative to adjacent normal tissues. Further investigation in the effect of miR-503 on ESCC cell proliferation, migration, and invasion showed that enhanced expression of miR-503 inhibited ESCC aggressive phenotype and overexpression of CCND1 reversed the effect of miR-503-mediated ESCC cell aggressive phenotype. Our study further identified CCND1 as the target gene of miR-503. Thus, miR-503 functions as a tumor suppressor and has an important role in ESCC by targeting CCND1.

Highlights

Peer review under responsibility of Beijing Institute of Genomics, Chinese Academy of Sciences and Genetics Society of China.Esophageal cancer (EC) is the fourth leading cause of cancerrelated death in China, and esophageal squamous cell carcinoma (ESCC) accounts for 90% of EC [1,2]
We examined the endogenous expression of miR-503 in 10 commonly-used ESCC cell lines, using the immortalized esophageal epithelial cell line NE2 as a control. qPCR analysis showed that compared to the NE2 cell line, miR-503 expression was markedly decreased in most of the ESCC cell lines examined (Figure 1A)
Our data showed that compared to the adjacent non-tumorous tissues from the same patient, there is a significant decrease in miR503 expression in the tumor samples from 83% of the ESCC patients (P = 0.0021, Figure 1B and C)

Summary

Introduction

Peer review under responsibility of Beijing Institute of Genomics, Chinese Academy of Sciences and Genetics Society of China. Esophageal cancer (EC) is the fourth leading cause of cancerrelated death in China, and esophageal squamous cell carcinoma (ESCC) accounts for 90% of EC [1,2]. Production and hosting by Elsevier B.V. on behalf of Beijing Institute of Genomics, Chinese Academy of Sciences and Genetics Society of China.

Methods

Results

Conclusion