Down-regulation of microRNA let-7d inhibits the proliferation and invasion of trophoblast cells in preeclampsia.

Abstract

Preeclampsia (PE) is a serious pregnancy complication that continues to adverse effects on mother and the fetus. The study investigated the effect of microRNA let-7d (miR-let-7d) on the proliferation and invasion of trophoblast cells (TC) in PE. In situ hybridization techniques were employed to evaluate the miR-let-7d expressions in the placental tissues from 63 PE patients as well as 65 normal placental tissues. Transfection of pre-let-7d, anti-let-7d, and their corresponding control sequences was performed in TCs obtained from PE. The blank group was composed of TCs no transfection and normal group consisted of normal TCs. Expressions of proliferation and invasion markers in TCs were detected by qRT-PCR. Proliferation, apoptosis, and the invasion ability of the TCs were determined using a CCK-8 assay, flow cytometry, and Transwell assay. In comparison to the normal placental tissues, PE placental tissues exhibited increased levels of let-7d expression. Following transfection of anti-let-7d, when compared with the blank group, the anti-let-7d group displayed increased levels of proliferation ability, expression of proliferation labeling proteins PCNA and Ki67, number of TCs as well as expressions of the invasion related proteins MMP-2, MMP-9, and TIMP-1. In contrast declines in cell apoptosis rate were observed. Compared with the blank group, the changes of the indexes were reversed in the pre-let-7d group. The study provided evidence suggesting that low expression levels of miR-let-7d plays a central role in suppressing apoptosis in addition to promoting the proliferation and invasion of PE TCs.