Downregulation of C12orf75 gene inhibits migration and invasion of liver cancer cell via suppressing the Wnt/β-catenin signaling pathway in vitro

Abstract

Hepatic carcinoma (HCC) is the second leading cause of death in cancer patients, and its incidence is on the rise. The prerequisite for the deterioration of liver cancer is the malignant migration and invasion of cancer cells, and in this study the C12orf75 gene was firstly showed that it has high expression characteristics in six HCC cell lines. By bioinformatics analysis, the up-regulation of C12orf75 was negatively correlated with the survival rate of HCC patients. CCK-8 and flow cytometry experiments indicated C12orf75 had no effect on cell proliferation, apoptosis and cycle of HuH-7 and Hep G2 cell lines, respectively. However, transwell assay showed that downregulation of C12orf75 gene could obviously inhibit invasion and migration of liver cancer cell. At the same time, this regulation process has been verified to involve the participation of Wnt/β-catenin signaling pathway. As a result, all these results showed that C12orf75 gene was a oncogene in liver cancer, which maybe a novel screening and diagnosing biomarker for patients with liver cancer, and this is of great significance for the development of targeted drugs for liver cancer patients.