Down-Regulation of Acid and Alkaline Phosphatases Induced After Prolonged Diclofenac Use in Experimental Mouse Bone

Abstract

The present investigation was undertaken to study the effect of diclofenac sodium on alkaline phosphatase and acid phosphatase activity in mice tibiae. Diclofenac was administered at the dose rate of 10 mg/kg body wt/day intramuscularly to experimental animals for 10, 20 and 30 days while the control group received normal saline. Alkaline phosphatase and acid phosphatase enzymes were assayed biochemically. Acid phosphatase activity was studied by histochemical analysis whereas alkaline phosphatase was analyzed by native PAGE. Student’s t test and 2-way Anova were applied wherever required. Significant decrease in acid phosphatase was revealed during this course of study which was recorded as 23.03, 22.06 and 15.72 % after 10, 20 and 30 days respectively. Similar trend of decreased acid phosphatase was demonstrated during histochemical study along with remarkable histopathological changes after diclofenac treatment for various durations of investigation. A down-regulation of 28.94 % was recorded in alkaline phosphatase activity of tibia after 10 days whereas no significant change was observed after 20 days. Slight increase (5.12 %) in alkaline phosphatase activity was documented after 30 days of drug therapy. Results from biochemical analysis of alkaline phosphatase were further supported by electrophoretic studies.