Downregulated long noncoding RNA ALDBGALG0000005049 induces inflammation in chicken muscle suffered from selenium deficiency by regulating stearoyl-CoA desaturase.

Ruifeng Fan,Jinxin Zhao,Qunxiang Shi,Shiwen Xu,Changyu Cao,Xia Zhao

doi:10.18632/oncotarget.17187

Abstract

Long non-coding RNAs (lncRNAs) have been demonstrated to play a pivotal role in proliferation and differentiation of muscles. However, the study on the roles of lncRNAs in Selenium (Se) deficiency induced muscle injury is still unclear. In this study, deep sequencing was performed to profile lncRNAs and mRNAs of the muscles from the Se deficiency (-Se group) and control (C group) chickens. The results revealed that 38 lncRNAs (23 up-regulated and 15 down-regulated) and 687 mRNAs (285 up-regulated and 402 down-regulated) were significantly dysregulated expressed, and the significantly dysregulated pathway including Phagosome, Cardiac muscle contraction, and Peroxisome Proliferator-Activated Receptor (PPAR) in -Se group. The regulatory relationship between ALDBGALG0000005049 and stearoyl-CoA desaturase (SCD), which involved in PPAR pathway was verified. The results also showed that the decreased expressions of SCD, PPARα, PPARβ and PPARγ, and the increased expressions of interleukin (IL)-1β, IL-6, IL-8, and chemokine (C-C motif) ligand 4 (CCL4) along with silencing of ALDBGALG0000005049 in chicken myoblasts. Moreover, increased expressions of IL-1β, IL-6, IL-8, and CCL4 and inflammatory cell infiltration in microstructure of chicken muscles treated with Se deficiency were observed. This study displayed an overview of aberrantly expressed lncRNAs and mRNAs profiles and PPAR pathway, and revealed that downregulation of ALDBGALG0000005049 caused inflammation by regulating SCD in chicken muscle resulted from Se deficiency.

Highlights

Se, as an essential micronutrient, plays an important role in various biological activities and Se deficiency induces some diseases including exudative diathesis [1], pancreatic atrophy [2], and nutritional muscular dystrophy [3] in chickens
We further explored the expression levels of IL-1β, IL-6, IL-8, and chemokine ligand 4 (CCL4) involved in inflammation induced by Se deficiency, and the results showed that protein expressions of IL-1β, IL-6, IL-8, and CCL4 were significantly increased (p
The results showed that 687 mRNAs and 38 Long non-coding RNAs (lncRNAs) were differentially expressed in -Se group compared with C group

Summary

Introduction

As an essential micronutrient, plays an important role in various biological activities and Se deficiency induces some diseases including exudative diathesis [1], pancreatic atrophy [2], and nutritional muscular dystrophy [3] in chickens. Recent studies have shown that dysregulated genes expression in selenoproteins [4], inflammation [5], apoptosis, and calcium signaling [6], which might contribute to Se deficiency-mediated nutritional muscular dystrophy. Long intergenic noncoding RNA, which is associated with muscle differentiation (linc-MD1) is greatly decreased in the muscles of Duchenne Muscular Dystrophy (DMD) patients, and overexpression of linc-MD1 can restore the defective myogenic differentiation [15]. Whereas long www.impactjournals.com/oncotarget noncoding RNA 31 expressed in several tissues including skeletal muscles, inhibits myogenic differentiation [16]

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Results

Conclusion