Down-regulation of TSGA10, AURKC, OIP5and AKAP4genes by Lactobacillus rhamnosus GG and Lactobacillus crispatus SJ-3C-US supernatants in HeLa cell line.

Abstract

Cancer testis antigens (CTAs) are considered cancer bio-markers due to their highly specific expression pattern in human malignancies and near absence from normal somatic tissues. Their specific expression has made them potential targets for early dia-gnosis, assessment of patients prognosis and treatment of cancer in recent years. Lactobacilli are agroup of probio-tics with anti-cancer, immunomodulatory and other beneficial features. These bacteria have been shown to alter expression of several cancer-related genes. We investigated the effect of Lactobacillus rhamnosus GG supernatant (LRS) and Lactobacillus crispatus SJ-3C-US supernatant (LCS) on expression of four CTAs (TSGA10, AURKC, OIP5and AKAP4) in HeLa cell line after synchronization using MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and quantitative real-time polymerase chain reaction. LRS and LCS inhibited HeLa cell growth after 24h as demonstrated by MTT assay. Expressions of all CTAs were down-regulated after treatment with both supernatants. This study showed the role of Lactobacilli in down-regulation of CTAs genes. Such expression change might be involved in the anticancer effects of these Lactobacilli. The underlying mechanisms of these observations are not clear but epigenetic modulatory mechanisms may participate in this process. Future studies are needed to assess functional roles of Lactobacilli in modulation of other cancer-related genes. Key words: probio-tic - cancer testis antigen - bio-marker - HeLa cell line.