Down-Regulation of SlGRAS10 in Tomato Confers Abiotic Stress Tolerance.

Sidra Habib,Yee Yee Lwin,Ning Li

doi:10.3390/genes12050623

Sidra Habib, Yee Yee Lwin + Show 1 more

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https://doi.org/10.3390/genes12050623

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Journal: Genes	Publication Date: Apr 22, 2021
Citations: 24	License type: CC BY 4.0

Affiliation: Chongqing University, Henan Normal University

Abstract

Adverse environmental factors like salt stress, drought, and extreme temperatures, cause damage to plant growth, development, and crop yield. GRAS transcription factors (TFs) have numerous functions in biological processes. Some studies have reported that the GRAS protein family plays significant functions in plant growth and development under abiotic stresses. In this study, we demonstrated the functional characterization of a tomato SlGRAS10 gene under abiotic stresses such as salt stress and drought. Down-regulation of SlGRAS10 by RNA interference (RNAi) produced dwarf plants with smaller leaves, internode lengths, and enhanced flavonoid accumulation. We studied the effects of abiotic stresses on RNAi and wild-type (WT) plants. Moreover, SlGRAS10-RNAi plants were more tolerant to abiotic stresses (salt, drought, and Abscisic acid) than the WT plants. Down-regulation of SlGRAS10 significantly enhanced the expressions of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) to reduce the effects of reactive oxygen species (ROS) such as O2− and H2O2. Malondialdehyde (MDA) and proline contents were remarkably high in SlGRAS10-RNAi plants. Furthermore, the expression levels of chlorophyll biosynthesis, flavonoid biosynthesis, and stress-related genes were also enhanced under abiotic stress conditions. Collectively, our conclusions emphasized the significant function of SlGRAS10 as a stress tolerate transcription factor in a certain variety of abiotic stress tolerance by enhancing osmotic potential, flavonoid biosynthesis, and ROS scavenging system in the tomato plant.

Highlights

Our study examined that the down-regulation of SlGRAS10 plants increased tolerance under abiotic stress by modulating the stress-inducible genes expression that are intricate in physiological modifications, such as flavonoid biosynthesis, reactive oxygen species (ROS) scavenging system, chlorophyll biosynthesis pathway and senescence associated genes
1), relate indicating that the SlGRAS10 belongs to the PAT1 subfamily and is more closely indicatingto that the
To study the likely physiological mechanism responsible for the enhanced salt and drought tolerance, we examined the alterations in MDA concentration, soluble sugar contents, and total proline contents from SlGRAS10RNAi and WT plants grown under control and abiotic stress

Summary

Introduction

Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Transcription factors are involved in plant growth. Transcription factors from the GRAS family were recognized based on DNA binding, transcriptional activation features, and nuclear localization [1]. The GRAS plant proteins are important for controlling several features of development, growth, and retorts to the abiotic and biotic conditions. The GRAS protein family name derived from its major three known participants, i.e., gibberellic acid insensitive (GAI), the repressor of GA1 (RGA), and a scarecrow (SCR) [2,3]. SCL14, a GRAS protein is known to be important for the stimulation of stress-inducible promoters in Arabidopsis thaliana [4]

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