Abstract
To investigate the expression level of TUG1 and one of its transcript variants (n377360) in osteosarcoma cells and assess the role of TUG1 in proliferation and apoptosis in the U2OS cell line. TUG1 and n377360 expression levels in patients with osteosarcomas and the U2OS human osteosarcoma cell line were evaluated using real-time quantitative PCR. U2OS cells were transected with TUG1 and n377360 siRNA or non-targeting siRNA. MTS was performed to assess the cell proliferation and flow cytometry was applied to analyze apoptosis. We found significantly higher TUG1 and n377360 expression levels in osteosarcoma tissues compared with matched non-tumorous tissues. In line with this, suppression of TUG1 and n377360 expression by siRNA significantly impaired the cell proliferation potential of osteosarcoma cells. Furthermore, inhibition of TUG1 expression significantly promoted osteosarcoma cell apoptosis. The overexpression of TUG1 and n377360 in osteosarcoma specimens and the functional role of TUG1 and n377360 regarding cell proliferation and apoptosis in an osteosarcoma cell line provided evidence that the use of TUG1 or n377360 may be a viable but an as yet unexplored therapeutic strategy in tumors that over express these factors.
Highlights
Osteosarcoma is the most common primary bone malignant tumor
The Taurine Up - regulated Gene 1 (TUG1) and n377360 expression levels were assessed in a group of 19 patients with osteosarcoma (Table 1)
The expression of TUG1 in osteosarcoma and matched small interfering RNA (siRNA). (B) MTS Cell proliferation assay of U2OS cell following nontumorous tissues. β-actin was used as an internal control. n377360 siRNA
Summary
Osteosarcoma is the most common primary bone malignant tumor. Osteosarcoma has lower incidence than many other malignant tumors, but it has high degree of malignancy, occurring mainly among children and adolescents with low 5-year survival rate, high amputation rate and poor postoperative function recovery (Qureshi et al, 2010). In-depth studies on gene regulation networks are essential for further understanding the mechanism governing the origination and development of osteosarcoma. Long non-coding RNAs (lncRNAs) are numerous, but the number of lncRNAs with known function is very small. Previous studies suggest TUG1 can regulate cell cycle by binding to PRC2. It has been reported over-expressed in bladder cancer (Khalil et al, 2009; Yang et al, 2011). We discussed the expression level of TUG1 and one of its different transcript variants in the sample including 19 cases of osteosarcoma tumor tissues and adjacent normal tissues. This study investigated the impact of TUG1 knockdown on the proliferation and apoptosis of bone sarcoma cell U2OS
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