Abstract
Long noncoding RNAs cancer susceptibility candidate 2 (CASC2) have been demonstrated as playing crucial regulatory roles in a few of cancers. However, the biological function of lncRNA CASC2 in bladder cancer are still unclear. In this study, we found that lncRNA CASC2 was significantly down-regulated in bladder cancer tissues and cell lines by quantitative real time-PCR and associated with advanced TNM stage (III/IV). Moreover, overexpression of lncRNA CASC2 remarkably reduced the cell growth, migration and invasion, as well as promoted early apoptosis of bladder cancer cell in vitro. Furthermore, we illustrated that lncRNA CASC2 inhibited Wnt/β-catenin signal pathway activity by decrasing the β-catenin expression and reversing the downstream target gene expression of Wnt signaling pathway. Taken together, lncRNA CASC2 plays an pivotal role in bladder tumorigenesis and progression and may act as a potential biomarker for the treatment of bladder cancer.
Highlights
Bladder cancer is one of the most prevalent urologic malignancy and the fourth more commonly diagnosed cancer among men worldwide [1]
We firstly explored the relative expression level of cancer susceptibility candidate 2 (CASC2) in bladder cancer tissues (n=72) compared with corresponding non-tumor tissues (n=72) by Quantitative real time RT-PCR (qRT-PCR), and normalized to GAPDH
We manifested that Long non-coding RNAs (lncRNAs) CASC2 expression was reduced in both bladder cancer tissues and cell lines and and negatively correlated with advanced TNM stage
Summary
Bladder cancer is one of the most prevalent urologic malignancy and the fourth more commonly diagnosed cancer among men worldwide [1]. There have been great improvements for treatment of bladder cancer, its great recurrence rate and extremely unsatisfactory prognosis with 5-year overall survival rates [4]. It is urgent need to perform molecular mechanisms research to discover novel molecular biomarkers for the treament of bladder cancer. Recent studies have showed that lncRNAs were tightly associated in carcinogenesis and can be used as the potential biomarkers of cancer [6]. LncRNAs, such as GAS5, UCA1, NEAT1, PANDAR, HOTAIR and H19 have been demonstrated that they showed crucial roles in the progression, metastasis, recurrence and prognosis of bladder cancer [7,8,9,10,11,12]. The functions of lncRNAs in bladder cancer remains largely unclear
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