Abstract
The diapause initiation and maintenance phases of the Colorado potato beetle, Leptinotarsa decemlineata, were screened. Eight transcripts were found to be downregulated as the beetles enter the diapause maintenance phase of diapause development after day 15 postemergence. These transcripts were also expressed in early nondiapausing adults. Using BlastX, the transcripts were placed into six broad categories: regulatory (serpin), structural (apidermin), protease (serine protease), retinol binding protein (CRALBP), carbohydrate metabolism (s-glucosidase, s-mannosidase, and cellulose II), and unknown function.
Highlights
The Colorado potato beetle, Leptinotarsa decemlineata (Say), is the major defoliator of potato throughout the northern hemisphere (Gauthier et al, 1981; Ferro, 1985; Weber & Ferro, 1994; EPPO, 2006)
Genes isolated in the unpublished investigation were subjected to more extensive developmental studies, revealing that eight genes were down-regulated as the beetles enter the maintenance phase (MP) of diapause
Northern blot screening of the diapause initiation and maintenance phase Transcript expression patterns were examined in days 1 to 14 initiation phase (IP) and days 15, 20, and 102 postemergence MP beetles
Summary
The Colorado potato beetle, Leptinotarsa decemlineata (Say), is the major defoliator of potato throughout the northern hemisphere (Gauthier et al, 1981; Ferro, 1985; Weber & Ferro, 1994; EPPO, 2006). By mid-September, the adults from the second generation burrow into the soil and enter diapause. Overwintering adults will spend approximately 9 months of their lives in diapause. Diapause is the physiological state of dormancy that enables insects to bridge periods of predictable seasonal adverse environmental conditions. Since favorable conditions necessary to enable reproduction and development are normally restricted to a narrow window of opportunity, insects spend most of their life cycle in diapause. Diapause has significant influence on other agriculturally important aspects of insect physiology. An interaction between diapause and the development of insecticide resistance in the Colorado potato beetle was discovered (Baker & Porter, 2008). Developing a more comprehensive understanding of diapause and its influence on insect life history traits will open up new avenues of pest management and offer insights into other biological processes (Denlinger, 2008)
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