Abstract
In our previous report, intracerebroventricular (i.c.v.) administration of leptin caused fat depletion by an induced adipocyte apoptosis in addition to influencing lipid metabolism. To uncover the biochemical mechanisms that mediate this response, the present study was designed to determine whether CCAAT/enhancer binding proteins (C/EBP)α, -β and -δ play a role in the leptin-induced fat depletion. Expressions of C/EBPα, -β and -δ in epididymal fat tissues were examined by Western immunoblot and in situ immunocytochemical analysis after 5 days of i.c.v. treatment. Young and old rats (3 and 8 months old) were treated with or without 5 μg/day leptin. The expression of C/EBPα, -β and -δ was decreased by i.c.v. leptin treatment in young rats as compared with controls ( P<0.05). However, leptin did not influence the expression of C/EBPα, -β and -δ in adipose tissues of 8-month-old rats. The basal level of expression of C/EBPβ was greater in 8-month-old rats than in 3-month-old rats, ( P<0.05) whereas the basal expression of C/EBPα and -δ was not different between age groups. These results were confirmed by in situ immunocytochemical analysis. The present study suggests that leptin-induced down-regulation of C/EBPα, -β and -δ might influence adipocyte differentiation and growth in a number of ways.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
