Down Regulation of Biofilm and Quorum Sensing Genes of Pseudomonas aeruginosa and Pasteurella multocida Isolated from Broiler Chicken Pericarditis Lesions by the Action of Some Essential Oils

Abstract

Pseudomonas and Pasteurella species have variant of virulence factors as biofilm genetic elements that form a thick coat surface surround pathogen and prevent its phagocytosis; also, quorum sensing genes which responsible for cell-to-cell communication and its inhibition are considered a new approach of antimicrobial therapy. The present investigation successes to identify of P. aeruginosa (85.2%) and P. multocida (41.8%) from broiler chickens pericarditis lesions by using Vitek2 compact system. Disc diffusion antibiogram analysis showed that P. aeruginosa were complete resistant to amoxicillin, kanamycin and lincomycin, while P. multocida show complete resistance to amoxicillin, doxycycline, gentamycin, florofenicol and lincomycin 30% of the tested isolates for biofilm formation by Congo red assay showed positive results. Down regulation of biofilm genes pslA, tadG and quorum sensing genes lasI, luxS in P. aeruginosa and P. multocida, respectively after exposure to 0.01% concentration of cinnamon and clove oils were detected by using RT-PCR.