Abstract
ObjectivesThe aim of this study was to investigate the effect of lncRNA-SNHG15 in bladder carcinoma using cell lines experiments and the relationship between clinical characteristics and lncRNA-SNHG15 expression was analyzed.MethodsBladder cancer tissues and near-cancer tissues were collected. The real-time PCR (RT-PCR) was used to detect the expression of lncRNA-SNHG15 in tissues and cell lines. The expression of lncRNA-SNHG15 was downregulated by interference (siRNA), as detected by RT-PCR, that was used to determine the efficiency of the interference. CCK-8 and Transwell assays were used to evaluate the effect of lncRNA-SNHG15 on the proliferation and invasion capability of bladder cancer cells. The t-test was used for Statistical analyses, which were carried out using the Statistical Graph pad 8.0.1.224 software.ResultThe expression of lncRNA-SNHG15 was up regulated in 5637, UMUC3 and T24 cell lines compared with corresponding normal controls (P < 0.05). Up regulation was positively related to tumor stage (P = 0.015). And tumor size (P = 0.0465). The down-regulation of lncRNA-SNHG15 with siRNA significantly inhibited UMUC3 and T24 cell proliferation and invasion.ConclusionThis study showed that lncRNA-SNHG15 is overexpressed in bladder cancer tissues and (5637, UMUC3 T24) cell lines. Up regulation was positively related to tumor stage (P = 0.015), and tumor size (P = 0.0465). Down-regulation of lncRNA-SNHG15 by siRNA significantly inhibited UMUC3 and T24 cell proliferation and invasion, indicating a potential molecular target for future tumor targeted therapy.
Highlights
Bladder cancer have a higher incidence in urinary malignancies
This study showed that Long non-coding RNA (lncRNA)-small nucleolar RNA host gene 15 (SNHG15) is overexpressed in bladder cancer tissues and (5637, UMUC3 T24) cell lines
We investigated the relationship between increased lncRNA-SNHG15 expression levels and clinical characteristics in 30 bladder cancer cases to see if lncRNA-SNHG15 expression is related to clinical features
Summary
Bladder cancer have a higher incidence in urinary malignancies. Because of its susceptibility to recurrence, progression, and metastasis, the current ideal treatment for bladder cancer is a comprehensive including surgery treatment, chemotherapy and Radiation therapy, but the overall effect is limited, and the invasion and metastasis are the main reasons of bladderMokhtar et al BMC Urol (2021) 21:8370% of the patients relapse within 5 years, and 10–30% of patients develop invasive urothelial cancer [2]. Non-coding RNA is a complex network of gene expression regulation that plays a key role in regulating many important biological functions of cancer cells. It is classified into two types based on the length of the sequence: short non-coding RNA and long non-coding RNA. Long non-coding RNA (lncRNA) is a class of transcripts with more than 200 nucleotides, and those without protein-coding functions are mostly transcribed by polymerase II [3]. The results showed that overexpression of lncRNA-SNHG15 was an associated molecular change in bladder carcinoma tissues, and cell lines (5637, UMUC3, T24). The effects of aberrant lncRNA-SNHG15 expression on the biological behavior of UMUC3 and T24 cell lines were investigated. The results provided novel insights into the function and mechanisms of lncRNA-SNHG15 bladder carcinoma pathogenesis, and lncRNA-SNHG15 was identified as a potential therapeutic target for cancer intervention
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