Abstract
OBJECTIVEMTDH has been identified to play a critical role in many cancer types, but its potential mechanism in Non‐Small Cell Lung Cancer (NSCLC) still remains unclear. We investigated the role of miRNA30a‐5p in the regulation of MTDH expression, which is known as an essential biomarker for NSCLC initiation and progression.MATERIALS AND METHODSQuantitative real‐time PCR (qRT‐PCR) has been performed to evaluate the expression level of MTDH and miR30a‐5p. Then, we transfected miR30a‐5p mimic and inhibitor into H1299 and A549 cells to investigate their impact in cell proliferation, migration, and invasion by using CCK‐8 assay, wound‐healing assay, and transwell assay respectively. The evidence of miR30a‐5p as a specific target of the MTDH gene was explored through luciferase report assay. The expression level of MTDH was evaluated by its mRNA and protein level using a western blot assay.RESULTSResults revealed that miR30a‐5p was upregulated whereas MTDH was downregulated. Down‐expression of miR30a‐5p increases the expression level of MTDH and promotes NSCLC cell proliferation and invasion. Luciferase report assay confirmed the binding of miR30a‐5p on MTDH mRNA 3′UTR region.CONCLUSIONSThis funding highlights the silencing mechanism of MTDH in lung cancer and further revealed that MTDH can be used as a potential diagnostic and therapeutic biomarker.
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