Abstract
After maintenance in a confluent state, human diploid fibroblasts were returned to a proliferative state by serial subcultivation. Their total metabolic time was greater than that of the continuously subcultivated controls. When the cells were serially subcultivated with low inoculation densities, their total metabolic time decreased considerably, while their total population doublings were slightly increased over cells split at 1:4 ratio. Thus, lifespan depends on the cumulative number of population doublings rather than on the total calendar time in vitro. The low-density inoculation method of the serial subcultivation is efficiently and economically advantageous in studying cellular aging in vitro.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
