Abstract
Ribonucleic acid (RNA) was extracted with phenol treatment from the rice dwarf virus (RDV) isolated from infected rice plants. This RNA has a complementary base composition, that is, both the ratios of (adenine/uracil) and (guanine/cytosine) are nearly unity. It contains 44% guanine plus cytosine. It appears as threads when precipitated in alcohol. A hyper-sharp schlieren pattern was obtained in the ultracentrifuge, as seen for DNA. The ultraviolet absorption of this RNA in 0.0015 M NaCl-0.00015 M sodium citrate showed a sharp increase when the temperature was about 80°. RDV-RNA did not react with formaldehyde at 37°, but after it was heated at 100° for 10 minutes and then cooled quickly it reacted with formaldehyde. RDV-RNA was resistant to ribonuclease digestion, as compared with ribosomal RNA or transfer RNA. It is concluded that the RNA of RDV is double-stranded.
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