Abstract
DNA polymerase plays an important role in the proliferation and survival of all forms of life and the variety of biotechnological applications. In this study, DNA‐templated copper nanoclusters were utilized to detect Klenow fragment exo‐ (KF‐) activity and monitor its inhibition in the presence of 5‐fluorouracil by measurement of fluorescence intensities. This study showed that the fluorescence intensities increased continuously for KF‐ concentrations ranging from 0 to 5 U/mL. In addition, inhibition of KF‐ activity was measured at 5‐fluorouracil concentrations ranging from 0 to 150 μM. Using nonlinear regression, the corresponding IC50 was determined to be 6.23 μM in the presence of 5.0 U/mL KF‐.
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