Abstract
In this work, a double signal amplified immunosensor based on the enhanced CdSe@ZnS quantum dots (QDs) electrochemiluminescence (ECL) via TiO2 nanoparticles (TiO2 NPs) and the outstanding quencher of polydopamine (PDA) decorated Au nanoparticles (Au@PDA NPs) for ultrasensitive detection of carcinoembryonic antigen (CEA) has been successfully achieved. The ECL of CdSe@ZnS QDs with different sizes has been investigated carefully, especially cooperation with TiO2 NPs. Au@PDA NPs have been synthesized and characterized by transmission electron microscopy (TEM) and UV–Vis spectrum, which acted as ECL quenchers to label the secondary antibody (Ab2) of CEA to form Ab2/Au@PDA NPs conjugates. The sandwich-structured immunosensor was formed between capture antibody (Ab1) on CdSe@ZnS QDs/TiO2 NPs/glassy carbon electrode, CEA and Ab2/Au@PDA NPs conjugates, resulting in a proportional ECL quenching signal relevant to the CEA concentration. Thus, CEA as a model biomarker has been detected in the linear range from 0.001 to 100ng mL−1 with a limit of detection of 0.35pg mL−1 (S/N = 3).
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