Abstract
The homonuclear scalar coupling of a directly bonded 13C– 13C pair has been used to create a double-quantum filter (DQF) to remove the natural-abundance 13C background in 13C{ 15N} rotational-echo double-resonance (REDOR) experiments. The DQF scalar and REDOR dipolar evolution periods are coincident which is important for sensitivity in the event of weak 13C– 15N dipolar coupling. Calculated and observed 13C{ 15N} DQF–REDOR dephasings were in agreement for a test sample of mixed recrystallized labeled alanines. Glycine metabolism in a single uniform- 15N soybean leaf labeled for 6 min by 13CO 2 was measured quantitatively by 13C{ 15N} DQF–REDOR with no background interferences.
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