Abstract
We present a new fiber-bundle-based endomicroscopy system to measure the fast cerebral blood flow (CBF) velocity in blood vessels located between the surface and the deep brain of living animals. The CBF velocity is obtained by measuring the displacement of the partially overlapped red blood cell images directly, using double-pulse 532-nm laser illumination. The proposed method could measure CBF in blood vessels with diameters ranging from 4 μm to 42 μm and could measure CBF velocities up to 3.2 μm/ms for different vessel diameters at a depth of 2.1 mm from the brain surface in a living mouse.
Highlights
Circulating cerebral blood transports products such as oxygen, nutrients, and blood-mediated messengers to brain and removes waste from the brain tissues, depending on the neuronal requirements [1]
We could observe the flow of red blood cells (RBCs) within capillaries of 9-μm diameter by visualizing the dark features, because the unlabeled RBCs appeared dark against the brighter background of the fluorescein isothiocyanate (FITC) dye mixed with the blood plasma, as shown in Fig. 3(A) and 3(B), and Visualization 1
These results demonstrate that the old optical platform using a single-pulse laser illumination could measure the slow blood flow
Summary
Circulating cerebral blood transports products such as oxygen, nutrients, and blood-mediated messengers to brain and removes waste from the brain tissues, depending on the neuronal requirements [1]. The exchange of these products between blood and brain tissue occurs at the microcirculation level. The measurement of the functional status and, in particular, the dynamics of the current microcirculatory network provides important information for researchers and clinicians. This is why various techniques are being developed to measure the spatiotemporal patterns of cerebral microcirculation
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