Abstract
Double-label analyses were performed to investigate somatostatin's coexistence with either enkephalin or gamma-aminobutyric acid (GABA) in amacrine cells of the chicken retina. Double-label immunocytochemistry revealed that although some amacrine cells labelled only for somatostatin or enkephalin, approx. 81% and 85% of somatostatin-immunopostive cells in the center and periphery of the retina, respectively, were also enkephalin-immunoreactive. Somatostatin-immunocytochemistry combined with autoradiography of high-affinity [ 3H]GABA uptake revealed that approx. 18% of somatostatin-immunoreactive amacrine cells exhibit high-affinity uptake of [ 3H]GABA.
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