Abstract
A double-isotope derivative technique has been developed for the selective assay of methylglyoxal in tissues. A measured amount of [1,3- 14C]methylglyoxal is added to the unknown sample and the bis-[3,5,6- 3H]2,4-dinitrophenylhydrazone is then prepared. The double-labeled derivative is purified by TLC, and both radioisotopes are counted. The ratio of 3H to 14C is a linear function of the amount of methylglyoxal in the sample. The lower limit for the analysis is 10 nmoles (0.7 μg) of methylglyoxal. In rat liver, no endogenous methylglyoxal was detected by this method. A previous report on the conversion of fructose 1,6-diphosphate into methylglyoxal was not substantiated.
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