Double insurance of paternity by a novel type of mating plug in a monandrous solitary mason beeOsmia bicornis(Hymenoptera: Megachilidae)

Abstract

Males of some hymenopteran species are able to behaviourally induce unreceptivity to mating in females by a post-copulatory display (post-copulatory courtship). This method of preventing further inseminations requires a high degree of female cooperation by the female and is especially susceptible to manipulations. If mating chances are low and sexual competition between males high, males can be expected to evolve additional mechanisms to protect their fertilization opportunities. This hypothesis was checked in the red mason bee Osmia bicornis (Linnaeus, 1758; syn. Osmia rufa). Males of this species were found to use a mating plug as a reinsurance to protect their paternity, although they induce females' monandry by a post-copulatory display. Male accessory gland secretions transferred during ejaculation form a plastic, spongy plug in the females' vagina that also contains the spermatozoa. This mating plug does not hinder rivals to mate with the female but prevents intermixing of the sperm. Thus, the sperm precedence of the first male is secured. The plug is ejected by the female after approximately 1 day when the spermatheca has been completely filled with sperm. The male accessory gland supply is sufficient for the formation of two to three plugs only and is not replenished. This pattern fits well with the low mating opportunities of O. bicornis males. The evolution of a mating plug in addition to the behavioural induction of unreceptivity to mate in females is discussed. © 2015 The Linnean Society of London, Biological Journal of the Linnean Society, 2015, 115, 28–37.