Abstract
目的 建立一种能快速有效分析阴沟肠杆菌是否产生超广谱β内酰胺酶(ESBLs)的方法.方法质控菌株为大肠埃希菌ATCC25922、肺炎克雷伯菌ATCC700603、阴沟肠杆菌029、阴沟肠杆菌O29M和阴沟肠杆菌1194E.用纸片双抑制剂平行抑制试验(DDIST)、临床和实验标准协会/美国临床实验标准化委员会(CLSI/NCCLS)推荐的用于检测大肠埃希菌、肺炎克雷伯菌ESBLs的纸片法和E-试验MIC法对本院连续的不重复的58株阴沟肠杆菌临床菌株进行ESBLs分析,用一系列ESBLs特异引物对其中27株耐头孢他啶或头孢噻肟的菌株进行扩增,以验证上述各种方法的准确性.结果PCR法从20/27株阴沟肠杆菌中扩增到ESBLs基因,DDIST法检出20株ESBLs产株,CLSI/NCCLS法仅检出4株,E-试验MIC法检出0株.结论纸片双抑制剂平行抑制试验是一种能快速有效分析阴沟肠杆菌超广谱β内酰胺酶的方法。
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