Abstract
Double-beam laser-excited indirect fluorometric detection of organic non-electrolytes is demonstrated in high-performance liquid chromatography. Anthracene is added as a visualization reagent in the mobile phase to maintain a background signal. When the analyte perturbs partitioning of the visualization reagent between the mobile and the stationary phases, it can be detected indirectly. A mass detectability of 50 ng of decanol is obtained (signal-to-noise ratio of 2) by using a microbore column.
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