Abstract
Double-antibody solid-phase (Dasp) method was applied to radioimmunoassays for follicle stimulating hormone (FSH) and luteinizing hormone (LH) in serum. The Dasp method uses second-antibody immunosorbent for the separation of free and antibody-bound antigen. Several parameters of the assays including concentrations of antiserum and second-antibody immunosorbent, reaction time and temperature were investigated. Reaction with second-antibody immunosorbent required only 2 hr at room temperature both in the FSH and LH assays. Nonspecific binding of labeled hormone to the immunosorbent was negligible. The FSH and LH assays were specific and sensitive enough to measure FSH and LH in serum. In these studies, the Dasp method proved to be useful for quantifying gonadotropins in large numbers of serum samples.
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