Abstract
An enzyme immunoassay (EIA) technique for plasma cortisol was Established by using cortisol-3 (carboxymethy1)oxime covalently linked To the horseradish peroxidase as the label antibody raised in the rabbits against cortisol -3- (carboxymethyly.)oxide -bovine serum Albumin was used as the first antibody .sheep ant-rabbit gamma-globulin Serum with & percent poly- ethyleneglycol were used to separate Antibody-bound and free cortisol. The enzyme activity of the bound fraction was measured with ortho-Phenylenediamine as substrate. the procedure performed at room Temperature was evaluated though sensitivity which was found to be 50Pg/tube .The correlation coefficient between our enzyme immunoassay Technique for determination of plasma
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