Abstract
A rapid and convenient procedure for the indirect serological detection of anti-Echinococcus antibodies in human hydatid disease is described. Sheep hydatid cyst fluid was spotted on nitrocellulose membranes. All remaining unbound sites on the membrane were blocked with polyxyethylene sorbitan monolaurate (Tween 20). The immobilized antigens were then reacted first with human serum to be diagnosed followed by reaction with horse anti-human IgG conjugated with peroxidase or protein A conjugated with peroxidase. The conjugated enzyme was then detected by the formation of the blue-brown precipitate which permanently stained the nitrocellulose membrane when the peroxidase reacted with benzidine-HCl and hydrogen peroxide. The results indicate that the dot-immunobinding assay is sensitive, specific, economical, fast and safe for serodiagnosis of hydatid disease. This is the first report of diagnosing human hydatid disease by the dot-immunobinding assay.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: The American journal of tropical medicine and hygiene
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
